Prep & Injection Guide

PEPTIDE STORAGE

Peptides are quite fragile, and require specific, stable storage conditions to retain their potency and maximize their shelf life. Whenever you purchase multiple vials of a peptide, you will only reconstitute the vial that you will be dosing from. The rest will be kept in either your refrigerator or freezer, depending on the period of time they will be stored prior to use.

Factors Influencing Peptide Stability

Temperature fluctuations: higher temperatures accelerate peptide degradation

Multiple freeze/thaw cycles: this is one of the fastest ways to diminish the efficacy of your peptides

Humidity: moisture can cause degradation and crystal formation. Even in their lyophilized state, peptides can absorb moisture from the air inside of the vial

Light exposure

Mechanical shock (when lyophilized vials are dropped or otherwise impact a hard surface with a decent amount of force): this can reduce efficacy and cause aggregation, particle formation, and cavitation (the formation and collapse of bubbles within a liquid)

Regardless of whether your peptide is reconstituted or lyophilized, and no matter if they will be stored in the refrigerator or freezer, you need an appropriate container to hold them.

  1. Get an opaque container which can be sealed tightly. If you do not have an opaque container, wrap your peptides in foil to prevent exposure to light.

  2. Consider purchasing desiccant packets — these are larger versions of the silica gel packets found in new shoes.

https://www.amazon.com/Wisesorb-Desiccant-Indicating-Silica-Packets/dp/B0C52NGYY3/

Place a couple silica packets into your storage container with your peptides to minimize moisture exposure. This may seem like overkill, but considering the detrimental effect moisture has on peptide efficacy, this extra step is worth the negligible cost.

  1. Tightly fasten the lid onto your container. Keep peptides in either the refrigerator or freezer according to storage duration.

Refrigeration of Reconstituted Peptides

For storage of reconstituted peptides for up to 60 days.

Place peptide vials into appropriate light-proof, airtight containers as specified above.

Do not store vials in the refrigerator door, as this subjects peptides to unnecessary agitation and damage.

If your dosing vial will last longer than 1–2 months and you are aliquoting reconstituted peptides for long-term freezer storage, use a buffer solution with a pH between 5–6 instead of bacteriostatic water. Aliquoting is transferring some of your reconstituted peptide into a sterile container, then storing it in the freezer. It is absolutely vital that this solution is frozen only once, as multiple freeze/thaw cycles are particularly damaging. Buffered solutions are available on Amazon.

Peptides stored in the refrigerator may be used for 60–90 days following reconstitution for most peptides (30–45 days for NAD+ and AOD-9604).

Lyophilized Powder Storage: Refrigerator

Exposure to bright artificial light or sunlight can degrade peptide quality.

Lyophilized peptides are stable at room temperature for 30–60 days. When not refrigerated or frozen, store them in a cool, dark, dry cupboard.

Lyophilized peptides intended for use within one year may be stored in the refrigerator. Store them in containers that block moisture and light.

For storage longer than one year, peptides should be kept in the freezer. When frozen, peptides may be stored for 2–3 years. Avoid multiple freeze/thaw cycles, as they cause rapid degradation.

For long-term use, consider a peptide vial storage container. Temperature-controlled options are ideal for travel and discretion. Both Etsy and Amazon offer suitable options.

Do not store Lipo-C in the refrigerator, as it will gel. Store between 68–77°F, away from light.




WHAT SUPPLIES WILL I NEED?

Alcohol prep pads.

Bacteriostatic water. Depending on peptide properties and intended use, other solvents such as saline solutions, specific buffers, or acetic acid may be required (e.g., acetic acid for AOD-9604). Necessary solutions are provided for all peptides in inventory.

Clean insulin syringes. It is advisable to understand your peptide dosing and purchase appropriately sized syringes. For single-digit unit dosing (e.g., 2, 3, 6 units), 0.3 mL (30-unit) syringes are recommended. Accurate measurement of small doses is nearly impossible with 0.5 mL or 1 mL syringes. Syringes are available from Amazon and ADW Diabetes.

Optional: 22 or 23 gauge, 3 mL syringe for reconstitution.

Optional: Light-blocking, moisture-resistant peptide storage container. Climate-controlled options are ideal for travel and discretion.




SYRINGES

Tip: The needle.
Barrel: The cylindrical part of the syringe with printed numbers.
Plunger: The movable part inside the barrel.

1 mL / 1 cc / 100-unit Syringe

Used for doses over 10 units.
Numbers reflect 10-unit graduations.
Each line between numbers equals TWO (2) units.
Single-unit dosing is not accurate.

0.3 mL / 0.3 cc / 30-unit Syringe

Used for single-unit dosing (2, 5, 7 units, etc.).
Numbers reflect 10-unit graduations.
Each line equals ONE (1) unit.
30 units are the same across syringe sizes; barrel size only affects appearance.
No dosing adjustment is required based on syringe size.

0.5 mL / 0.5 cc / 50-unit Syringe

Suitable for single-unit dosing, especially for doses under 10 units.
Numbers reflect 10-unit graduations; each line equals ONE (1) unit.

10 mL Luer Lock Syringe

Used for Lemon Bottle administration or large-volume use.
Does not include a needle.
Allows use of different needle sizes for drawing and injecting.
Dosing needles can be used for both.

Luer Lock Needle Hub

Twists onto the Luer Lock barrel.
Often sold separately.
Available in various gauges and lengths.

3 mL Reconstitution Syringe

Numbered lines indicate 0.5 mL increments.
Short lines indicate 0.1 mL increments.
Used for accurate peptide reconstitution.

Needle Gauge

Needle gauge refers to thickness (higher number = thinner needle).
Most dosing syringes are 29–31 gauge.
Reconstitution syringes are typically 27 gauge.

Standard Syringe Lengths for Subcutaneous Injection

Common lengths: 5/16″ (8 mm) and ½″ (12 mm).
½″ is the maximum recommended length for subcutaneous use.




HOW TO ORDER DOSING SYRINGES

  1. Review your dosing instructions.
    Determine whether you need 30-, 50-, or 100-unit syringes.

Single-unit dosing requires 3/10 mL or 0.5 mL syringes.
Doses over 10 units may use 1 mL syringes.

  1. Interpreting syringe labels.
    Example: “29 gauge, 1 cc, ½″ (12 mm)”

29 gauge: acceptable dosing size.
1 cc: equals 1 mL or 100 units.
½″ (12 mm): appropriate for subcutaneous and intramuscular dosing.

  1. Recommended syringes.

Single-unit dosing:
30–31 gauge, 3/10 mL (30-unit) syringes with 5/16″ tips.

Doses over 10 units:
29–31 gauge, 1 mL syringes with 5/16″ tips, or
29–31 gauge, 1 mL syringes with ½″ tips for mixed dosing.




RECONSTITUTION

Remove plastic caps from the peptide vial and bacteriostatic water.

Clean both vial tops with an alcohol prep pad.

Select a reconstitution syringe (22–23 gauge, 3 mL preferred).

Insert syringe into BAC water, invert vial, and draw required volume.

Inject water slowly into the peptide vial.

Remove syringe and dispose of it.

Gently roll vial between palms to mix.
Allow to sit 15–30 minutes to complete dissolution.

Peptide is now ready for use.
Roll gently before each dose and return to refrigerator after use.




INJECTION GUIDELINES

Subcutaneous

Clean peptide vial top.
Remove syringe caps.
Draw prescribed dose (inject air if flow is slow).
Clean injection site.
Pinch skin, insert needle fully, inject dose.
Wait a few seconds, remove and dispose syringe.
Rub site for one minute.
Return peptide to refrigerator.

Best locations for subcutaneous injections are shown in the diagram.

Intramuscular

Clean vial top.
Draw dose.
Clean injection site.
Insert needle into muscle quickly.
Inject dose fully, wait briefly, then remove syringe.
Rub site for one minute.
Return peptide to refrigerator.

Best locations for intramuscular injections are shown in the diagram.

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